Transition of haemoglobin between two tertiary conformations: Inositol hexakisphosphate increases the transition constant and the affinity of sheep haemoglobin for 5,5′-dithiobis(2-nitrobenzoate)

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The equilibrium constant (Kequ) for the reaction of 5,5′-dithiobis(2-nitrobenzoate) — DTNB — with the CysF9 [93]β sulphydryl group of the haemoglobins of the sheep decreases by about two orders of magnitude between pH≈5.6 and 9.2: from a mean of 7.2±1 to a mean of 0.044±0.01. Calculations from the pH dependence of Kequ show that in the r⇌t tertiary conformational transition of...

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KO Okonjo, I. Adeogun & JO Babalola

Okonjo Kehinde » Born 1942. Educated King's College, Lagos (1957-63). BSc, PhD (University of Ibadan). Retired Professor of Chemistry, University of Ibadan. Currently Professor of Chemistry, Covenant University. Current research interests: Kinetics and thermodynamics of reactions of reaction of Ellman's reagent with hemoglobin sulphydryl groups. Publications in such high impact journals as the Journal of... view full profile

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