Reversible reaction of 5,5′-dithiobis(2-nitrobenzoate) with the CysF9[93]β sulfhydryl groups of the hemoglobins of the domestic cat: Variation of the equilibrium and reverse rate constants with pH

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We have determined for the first time the equilibrium constant, Keq, for the reaction of Ellman's reagent, 5,5′-dithiobis(2-nitrobenzoate), with the CysF9[93]β sulfhydryl groups of the hemoglobins of the domestic cat. In the pH range 5.6 to 9.0 Kequ varies over four orders of magnitude — between ca 10 and 10−3 — for all hemoglobin derivatives. Using these Kequ values and published...

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KO Okonjo, AA Fodeke & AT Kehinde

Okonjo Kehinde » Born 1942. Educated King's College, Lagos (1957-63). BSc, PhD (University of Ibadan). Retired Professor of Chemistry, University of Ibadan. Currently Professor of Chemistry, Covenant University. Current research interests: Kinetics and thermodynamics of reactions of reaction of Ellman's reagent with hemoglobin sulphydryl groups. Publications in such high impact journals as the Journal of... view full profile

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